Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF Summary

• Additional Information: His10 Neddylated
• Details of Functionality: Typical concentration to support in vitro conjugation reactions will depend on experimental conditions.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1/DDB1/CRBN Complex protein
  This complex consists of an N-terminal 10-His tagged, neddylated Cullin-4A , untagged RBX1, untagged DDB1, and untagged CRBN.
• Accession #: Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1)
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 104 kDa (Neddylated CUL4A), 12 kDa (RBX1), 127 kDa (DDB1), 51 kDa (CRBN).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

• CUL4A/RBX1/DDB1/CRBN Complex

Background

Cullin-4A is a core component of multiple cullin-RING type ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-Cul4-x-box), Cullin-4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the Rbx1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on the neddylation of the cullin subunit, though neddylation may be dispensable in some in vitro reactions.  

1. Fischer, E.S., et al.  (2014) Nature  512:49.
2. He, Y.J., et al.  (2006) Genes Dev.  20:2949.
3. Ito, T., et al.  (2010) Science  327:1345.
4. Wang, H., et al. (2006) Mol. Cell  22:383.

Bioinformatics

• Uniprot:
  • Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1)()