Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF Summary

• Additional Information: His-tag
• Details of Functionality: Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL2/RBX1 Complex protein
  Met1 - Ala745 with a N-terminal 10-His Sumo tag (CUL2), Met1 - His108 (RBX1)
• Accession #: Q13617.2 (CUL2), P62877.1 (RBX1)
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 102 kDa (neddylated CUL2), 12 kDa (RBX1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF

• CUL2/RBX1 Complex

Background

Cullin-2 is a core component of multiple cullin-RING based "ECS" (Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in a variety of cellular processes.  In the ECS complex, Cullin-2 serves as a scaffold that organizes the ELOB/C-associated recognition subunits with the RBX1 subunit and contributes to the catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. 

1. Baek K., et al. (2020) Nature 578: 461.
2. Duda D.M., et al. (2008) Cell  134: 995.
3. Duda D.M., et al. (2012) Mol. Cell  47: 371.
4. Goldenberg S.J., et al. (2004) Cell  119: 517.
5. Zheng N., et al. (2002) Nature  416: 703.

Bioinformatics

• Uniprot:
  • Q13617.2 (CUL2), P62877.1 (RBX1)()