Recombinant Human CUL1/RBX1 Neddylated Complex Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CUL1/RBX1 Neddylated Complex Protein, CF Summary

• Details of Functionality: Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/RBX1 Complex protein
  Met1 - Ala776 with a N-terminal 10-His Sumo tag (CUL1), Met1 - His108 (RBX1)
• Accession #: Q13616.2 (CUL1), P62877.1 (RBX1)
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 104 kDa (neddylated CUL1), 12 kDa (RBX1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CUL1/RBX1 Neddylated Complex Protein, CF

• CUL1/RBX1 Complex

Background

Cullin-1 (CUL1) is a core component of multiple SCF (SKP1-CUL1-F-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  In the SCF complex, CUL1 serves as a scaffold that organizes the SKP1-F-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

1. Baek K., et al. (2020) Nature 578: 461.
   
2. Duda D.M., et al.  (2008) Cell 134: 995.
3. Duda D.M., et al.  (2012) Mol. Cell 47: 371.
4. Goldenberg S.J., et al.  (2004) Cell  119: 517.
5. Zheng N., et al.  (2002) Nature 416: 703.

Publications for CUL1/RBX1 Complex (E3-411)(1)

Publication using E3-411

• Hong, SH;Osa, A;Wertz, IE;Nomura, DK; Exploiting the Cullin E3 Ligase Adaptor Protein SKP1 for Targeted Protein Degradation bioRxiv : the preprint server for biology 2023-10-21 [PMID: 37904950] (Bioassay, N/A)

Bioinformatics

• Uniprot:
  • Q13616.2 (CUL1), P62877.1 (RBX1)()