Recombinant Human CLF-1/CLC Complex Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human CLF-1/CLC Complex Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells transfected with human CNTF R alpha . The ED 50 for this effect is 0.5-3.5 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human CLF-1/CLC Complex protein

  Human CLF-1 (Ala38 - Arg422) Accession # O75462.1

  10-His tag

  Human CLC (Leu28 - Phe225) Accession # Q9UBD9

  6-His tag

  N-terminus

  C-terminus

• Accession #: O75462 (CLF-1) & Q9UBD9 (CLC)
• N-terminal Sequence: Ala38 (CLF-1) & Leu28 (CLC)
• Protein/Peptide Type: Recombinant Proteins
• Gene: CRLF1
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44.5 kDa (CLF-1) & 23 kDa (CLC).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CLF-1/CLC Complex Protein

• CLF-1/CLC Complex

Background

Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor1 (CLF-1), a member of the Cytokine Type I Receptor family, to form the heteromeric composite cytokine CLC/CLF-1 (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha ) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3 - 5). CLC/CLF-1 binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate thePI 3-kinase and the MAP kinase activity. The CLC/CLF-1 complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Human and mouse CLF-1 share 96% amino acid sequence identity (3). Human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence homology. Human CLC also shares 96% amino acid sequence homology with mouse CLC. CLC/CLF-1 supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4 - 6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).

1. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
2. Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
3. Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371.
4. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
5. Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867.
6. Uemura, A. et al. (2002) Cytokine 18:1.
7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
8. Senaldi, G. et al. (2002) J. Immunol. 168:5690.

Publications for CLF-1/CLC Complex (1151-CL)(3)

Publications using 1151-CL

• Kass DJ, Yu G, Loh KS, Savir A, Borczuk A, Kahloon R, Juan-Guardela B, Deiuliis G, Tedrow J, Choi J, Richards T, Kaminski N, Greenberg SM Cytokine-like factor 1 gene expression is enriched in idiopathic pulmonary fibrosis and drives the accumulation of CD4+ T cells in murine lungs: evidence for an antifibrotic role in bleomycin injury. Am. J. Pathol., 2012-03-16;180(5):1963-78. 2012-03-16 [PMID: 22429962] (Bioassay, In Vivo, Mouse, Rat)
• Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer. Mol. Cell. Biol., 2010-06-28;17(0):4175. 2010-06-28 [PMID: 20584990] (Bioassay, Surface Plasmon Resonance, Human)
• Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002. 2005-05-25 [PMID: 15917337] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: CRLF1
• Uniprot:
  • O75462 (CLF-1) & Q9UBD9 (CLC)()