Recombinant Human CLEC2L Fc Chimera Protein, CF

When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Galectin-3 (Catalog # 1154-GA) binds with an ED50 of 1.5-12 μg/mL.

2 μg/lane of Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-44 kDa and 80-90 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CLEC2L Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin-3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is 1.5-12 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human CLEC2L protein
  

  MD	Human IgG2A (Pro100-Lys333)	IEGR	Human CLEC2L (Ser90-Thr214) Accession # P0C7M8-1
  N-terminus			C-terminus

• Accession #: P0C7M8-1
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-44 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CLEC2L Fc Chimera Protein, CF

• CLEC2L
• C-type lectin domain family 2 member L
• C-type lectin domain family 2, member L

Background

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

1. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
2. Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
4. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
5. Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
6. Lysenko, O. et al. (2013) PloS One. 8:e65345.

Bioinformatics

• Uniprot:
  • P0C7M8-1()