Recombinant Human CEACAM-20 His-tag Protein, CF

Immobilized Recombinant Human CEACAM-20 His-tag (Catalog # 10277-CM) enhances L cells mouse fibroblast cell line adhesion. The ED50 for this effect is 0.25‑1.5 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CEACAM-20 His-tag Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED₅₀ for this effect is 0.25-1.5 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human CEACAM-20 protein
  Gln31-Gly450, with a C-terminal 6-His tag
• Accession #: Q6UY09-1
• N-terminal Sequence: Gln31 inferred from enzymatic pyroglutamate treatment revealing Leu32
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 73-84 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-20 His-tag Protein, CF

• CEACAM20
• CEACAM-20
• GPAD9366
• UNQ9366

Background

Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 amino acid (aa) extracellular domain (ECD), a 21 aa helical transmembrane segment, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). Within the ECD, human CEACAM-20 shares 64% and 62% aa identity with the mouse and rat CEACAM-20, respectively. The cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought to act as a physiological substrate for SAP-1 in the intestinal epithelium (10).

1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
2. Zhang, H. et al. (2013) PLoS ONE 8:e53359.
3. Zebhauser, R. et al. (2005) Genomics 86:566.
4. Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
5. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
6. Murata, Y. et al. (2015) PNAS E4264.
7. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
8. Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283.
9. Kitamura, Y. et al. (2015) Genes to Cells 20:578.
10. Kotani,  T. et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.

Bioinformatics

• Uniprot:
  • Q6UY09-1()