Recombinant Human CCL23/MPIF-1 Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human CCL23/MPIF-1 Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.02-0.1 µg/mL.
• Source: E. coli-derived human CCL23/MPIF-1 protein
  Arg22-Asn120
• Accession #: P55773
• N-terminal Sequence: Arg22
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL23
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 11.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL23/MPIF-1 Protein

• CCL23
• CCL23/MPIF-1
• CCL23;CKb8;Ckb-8;Ckb-8-1;CK-BETA-8;hmrp-2a;MIP3;MIP-3;MPIF1;MPIF-1;SCYA23
• MPIF1
• MPIF-1

Background

Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.

1. Patel, V. et al. (1997) J. Exp. Med. 185:1163.
2. Youn, B-S. et al. (1998) Blood 91:3118.
3. Nardelli, B. et al. (1999) J. Leukoc. Biol. 61:822.
4. Berkhout, T.A. et al. (2000) Biochem. Pharmacol. 59:591.

Publications for CCL23/MPIF-1 (371-MP)(4)

Publications using 371-MP

• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Kim J, Kim YS, Ko J CK beta 8/CCL23 induces cell migration via the Gi/Go protein/PLC/PKC delta/NF-kappa B and is involved in inflammatory responses. Life Sci., 2009-12-04;86(9):300-8. 2009-12-04 [PMID: 19951712] (Bioassay, Human)
• Miao Z, Premack BA, Wei Z, Wang Y, Gerard C, Showell H, Howard M, Schall TJ, Berahovich R Proinflammatory proteases liberate a discrete high-affinity functional FPRL1 (CCR12) ligand from CCL23. J. Immunol., 2007-06-01;178(11):7395-404. 2007-06-01 [PMID: 17513790] (Bioassay, Human)
• Novak H, Muller A, Harrer N, Gunther C, Carballido JM, Woisetschlager M CCL23 expression is induced by IL-4 in a STAT6-dependent fashion. J. Immunol., 2007-04-01;178(7):4335-41. 2007-04-01 [PMID: 17371990] (ELISA (Standard))

Bioinformatics

• Gene Symbol: CCL23
• Uniprot:
  • P55773()