Recombinant Human CCL23/MPIF-1 (aa 46-120) Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL23/MPIF-1 (aa 46-120) Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.70-3.5 ng/mL. This 46‑120 aa variant of CCL23 is significantly more active than the 22‑120 aa variant (Catalog # 371-MP). The ED₅₀ of the 22‑120 aa variant on hCCR1 transfected BaF3 cells is 20-100 ng/mL.
• Source: E. coli-derived human CCL23/MPIF-1 protein
  Arg46-Asn120
• Accession #: P55773.2
• N-terminal Sequence: Arg46
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL23
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL23/MPIF-1 (aa 46-120) Protein, CF

• CCL23
• CCL23/MPIF-1
• CCL23;CKb8;Ckb-8;Ckb-8-1;CK-BETA-8;hmrp-2a;MIP3;MIP-3;MPIF1;MPIF-1;SCYA23
• MPIF1
• MPIF-1

Background

CCL23, also known as MPIF-1, Ck beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the CCL23 gene results in two mRNAs that encode a short (Ck beta 8) and a long (Ck beta 8-1) isoform of the chemokine. Ck beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature Ck beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue Ck beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, Ck beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, CCL23 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). CCL23 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. CCL23 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. CCL23 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, osteoclast precursors.

1. Patel, V. et al. (1997) J. Exp. Med. 185:1163.
2. Youn, B-S. et al. (1998) Blood 91:3118.
3. Nardelli, B. et al. (1999) J. Leukoc. Biol. 61:822.
4. Berkhout, T.A. et al. (2000) Biochem. Pharmacol. 59:591.

Publications for CCL23/MPIF-1 (131-M1/CF)(1)

Publication using 131-M1/CF

• Miao Z, Premack BA, Wei Z, Wang Y, Gerard C, Showell H, Howard M, Schall TJ, Berahovich R Proinflammatory proteases liberate a discrete high-affinity functional FPRL1 (CCR12) ligand from CCL23. J. Immunol., 2007-06-01;178(11):7395-404. 2007-06-01 [PMID: 17513790] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CCL23
• Uniprot:
  • P55773.2()