Recombinant Human C1qTNF11/C1qL4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human C1qTNF11/C1qL4 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL4 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera (Catalog # 9338-BA) that produces 50% of the optimal binding response is 2-10 μg/mL.
• Source: E. coli-derived human C1qL4 protein
  Gly105-Asp238, with an N-terminal Met and 6-His tag
• Accession #: Q86Z23
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 13 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human C1qTNF11/C1qL4 Protein, CF

• C1qL4
• C1qTNF11
• complement C1q-like protein 4
• complement component 1, q subcomponent-like 4
• CTRP11
• MGC131708

Background

C1qTNF11 (CTRP11), also known as C1qL4, is an approximately 29 kDa member of the C1qTNF family of secreted proteins (1, 2). Mature human C1qTNF11/C1qL4 contains two distinct domains: a collagen-like region and one C1q-like domain, and can form disulfide-linked heteromers with C1qTNF14/C1qL1 (3, 4). The C1qTNF11/C1qL4 gene is conserved in human, chimpanzee, dog, mouse, rat, zebrafish, and frog. Within the C1q-like domain, human C1qTNF11/C1qL4 shares 100% amino acid (aa) sequence identity with that of mouse, monkey and rat. C1qTNF11/C1qL4 is expressed predominantly in testis and adipocytes, and also at a lower level in skeletal muscle and kidney (4), hippocampus, and cerebral cortex (5). Similar to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF14/C1qL1, C1qTNF11/C1qL4 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF11/C1qL4 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7). Our in house assay shows that C1qTNF11/C1qL4 also binds to BAI-2.  In addition, C1qTNF11/C1qL4 has been reported to promote angiogenesis via activation of the ERK pathway in HUVEC cells mediated by BAI3 (9). Conversely, in adipocytes, C1qTNF11/C1qL4 treatment decreased ERK1/2 signaling and inhibited adipogenesis (4).

1. Ghebrehiwet, B. et al. (2012) Front. Immunol. 3: 52.
2. Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
3. Ressl, S. et al. (2015) Structure 23:688.
4. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
6. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
7. Kakegawa, W. et al. (2015) Neuron 85:316.
8. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
9. Liu, F. et al. (2017) Mol. Cell. Biochem. 424:57.

Bioinformatics

• Uniprot:
  • Q86Z23()