Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) iscoated onto a microplate at 2 µg/mL, Recombinant Human APP695 Fc Chimera binds with an ED50 of 7-42 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Amyloid Precursor Protein Fc Chimera, CF Summary

• Additional Information: Isoform APP695
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated at 2 µg/mL, 100 μL/well, Recombinant Human APP695 Fc Chimera binds with an ED₅₀ of 7-42 ng/mL
• Source: Mouse myeloma cell line, NS0-derived human APP695 protein
  

  Human Amyloid-beta A4 (Isoform APP695) (Leu18-Lys612) Accession # P05067-4	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P05067-4
• N-terminal Sequence: Leu18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 111-133 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

• APP695

Background

Amyloid precursor protein (APP) is a type I membrane protein with several isoforms due to alternative splicing. Of the three major splice isoforms of APP (APP695, APP751, and APP770) APP695 is the predominant neuronal form from which Amyloid beta peptide  and transcriptionally-active cleaved intracellular domain of APP (AICD) are preferentially generated by selective processing through the amyloidogenic pathway (1). Human APP695 consists of a 17 amino acid (aa) signal sequence, a 607 aa extracellular domain (ECD), a 24 aa transmembrane domain, and a 47 aa cytoplasmic domain. Within the ECD, human APP695 shares 97% aa sequence identity with mouse and rat APP695. Amyloid beta is a major molecule implicated in pathogenesis of Alzheimer's disease (AD) and related dementias (2). AICD regulates expression by direct promoter binding of multiple genes, including APP itself, the beta-secretase, BACE-1 and the Amyloid beta-degrading enzyme, Neprilysin (3, 1). As such, APP695 plays an important role in brain development, learning and memory, synaptic plasticity, and neurodegeneration including AD (4).

1. Nalivaeva, N.N. and Turner A. J. (2013) FEBS Lett. 587:2046.
2. Haass, C. (2004) EMBO J. 23:483.
3. Passer, B. et al. (2000) J. Alzheimers Dis. 2:289.
4. Benilova, I. et al. (2012) Nat. Neurosci. 15:349.

Bioinformatics

• Uniprot:
  • P05067-4()