Recombinant Human Activin AB Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Activin AB Protein Summary

Details of Functionality
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2‑1.2 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Activin AB protein
Human Activin beta A
(Gly311 - Ser426)
Accession # NP_002183.1
Human Activin beta B
(Gly293 - Ala407)
Accession # NP_002184.2
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly311( beta A subunit) & Gly293 ( beta B subunit)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.8 kDa ( beta A subunit) and 13 kDa ( beta B subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Publications
Read Publications using
1066-AB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Activin AB Protein

  • Activin AB

Background

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned. The nomenclature reflects the subunit composition of the proteins: Activin A ( beta A - beta A), Activin B ( beta B - beta B), and Activin AB ( beta A - beta B). Activin A, Activin B, and Activin AB are present in gonadal tissues and are biologically active proteins. However, little is known about the contribution of the other beta subunits to Activin formation and function since knock-outs of beta C and beta E in mice do not exhibit a phenotype.

At the amino acid sequence level, the mature human beta A subunit is 100% identical to mouse beta A, while the mature human and mouse beta B subunits share 98% identity. The mature beta A and beta B subunits share less than 80% amino acid identity. Mice with targeted mutations of beta A, beta B, or both genes do not show mesodermal defects, indicating Activin is not involved in mesoderm formation in mammals as it is in Xenopus. Also, the double homozygous mutants have the whole spectrum of defects associated with either mutation alone, suggesting that Activin beta A and beta B do not compensate for one another, nor do they have overlapping functions. Similar to other TGF-beta family members, Activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II. Activin binds directly to ACT RII, the complex then associates with ACT RI and initiates signaling through the SMADs.

  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
  3. Chang, H. et al. (2001) Mol. Cell. Endocrinology 180:39.

Publications for Activin AB (1066-AB)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance.


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Bioassay
(3)
Surface Plasmon Resonance
(2)
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Human
(3)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 5 of 5.
Publications using 1066-AB Applications Species
BC Goh, V Singhal, AJ Herrera, RE Tomlinson, S Kim, MC Faugere, EL Germain-Le, TL Clemens, SJ Lee, DJ DiGirolamo Activin Receptor Type IIA (ACVR2A) Functions Directly in Osteoblasts as a Negative Regulator of Bone Mass J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28659341] (Bioassay, Mouse) Bioassay Mouse
E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada Activin A more prominently regulates muscle mass in primates than does GDF8 Nat Commun, 2017;8(0):15153. 2017 [PMID: 28452368] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta. PLoS ONE, 2015;10(2):e0118097. 2015 [PMID: 25707005] (Bioassay, Human) Bioassay Human
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Maeshima A, Vaughn DA, Choi Y, Nigam SK Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev. Biol., 2006;295(2):473-85. 2006 [PMID: 16643884] (Bioassay, Rat) Bioassay Rat

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