Recombinant HIV-1 Gag p24 His-tag Protein, CF

2 μg/lane of Recombinant HIV-1 Gag p24 His-tag Protein (Catalog # 11243-HV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-25 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant HIV-1 Gag p24 His-tag Protein, CF Summary

• Additional Information: E. coli-expressed
• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived viral HIV-1 Gag p24 protein
  Pro149-Gly354, with a C-terminal 6-His tag and a N-terminal Met
• Accession #: NP_057850.1
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 21-25 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, Urea with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant HIV-1 Gag p24 His-tag Protein, CF

• CA
• Capsid Protein
• HIV1 Gag p24
• HIV-1 Gag p24
• Pr55(Gag)

Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load. This product is intended to be used to generate standard curves and/or positive controls for use in HIV-1 Gag p24 immunoassays.

Bioinformatics

• Uniprot:
  • NP_057850.1()