Recombinant His6-SARS Virus Papain-like Protease Protein, CF

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Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant His6-SARS Virus Papain-like Protease Protein, CF Summary

Details of Functionality
Recombinant SARS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate.  Using tetra-Ubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages when using a 100 nM enzyme concentration--at higher enzyme concentration other linkages are cleaved as well.
Source
E. coli-derived viral Papain-like Protease protein
MHHHHHHGENLYFQSG, Glu1541 - Lys1841
Accession #
Protein/Peptide Type
Recombinant Enzymes
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
E-610 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

  • Papain-like Protease
  • PLpro
  • PL-PRO
  • pp1a
  • Replicase polyprotein 1a

Background

The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.  PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro.  When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species.  This protein contains an N-terminal 6-His tag.
  1. Frieman M., et al.  (2009) J.Virol.  83: 6689
  2. Lindner H.A.,et al.  (2007) Archv. Biochem. Biophys.  466: 8
  3. Ratia K., et al.  (2014) PLoS Pathog  doi: 10.1371/journal.ppat.1004113
  4. Russell N., et al. (2016) FASEB Ubiquitin & Cell Reg.  (poster)
 

Publications for Papain-like Protease (E-610)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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