Recombinant A. caninum NIF Protein, CF

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Product Details

Summary
Reactivity AcSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant A. caninum NIF Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils. The ED50 for this effect is 0.1-0.4 μg/mL. Also measured by its ability to bind Recombinant Human Integrin  alpha M beta 2 (Catalog # 4047-AM) in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived a. caninum NIF protein
Asn18-Leu274, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asn18
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-65 kDa, reducing conditions
Publications
Read Publications using
5845-NF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant A. caninum NIF Protein, CF

  • NIF

Background

Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host (1, 2). NIF interacts with the leukocyte integrin alpha M beta 2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium (1). The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites (1). Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation‑dependent, reversible inhibition of alpha M beta 2-mediated adhesion and degranulation (1 - 4). The NIF binding site within the alpha M beta 2 I-domain interferes with activation‑dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen (2, 3). In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury (4, 5).

  1. Moyle, M. et al. (1994) J. Biol. Chem. 269:10008.
  2. Rieu, P. et al. (1994) J. Cell Biol. 127:2081.
  3. Ustinov, V.A. and E.F. Plow (2002) J. Biol. Chem. 277:18769.
  4. Barnard, J.W. et al. (1995) J. Immunol. 155:4876.
  5. Zhou, M.-Y. et al. (1998) J. Clin. Invest. 101:2427.

Publications for NIF (5845-NF)(2)

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Other Available Formats

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Array 5845-NF

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Bioinformatics

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