Real Blue Peroxidase Substrate Kit

Immunohistochemistry-Paraffin: Real Blue Peroxidase Assay Kit [KA0565] - IHC-P staining of epithelial membrane antigen in kidney tissue using chromogen reagent Real Blue Peroxidase Assay Kit (KA0565)

Product Discontinued

Real Blue Peroxidase Substrate Kit Summary

• Description: Quality control test: IHC demonstration data
  
  Materials Required but Not Supplied: Primary antibody; HRP labeled reagent; Isopropyl alcohol; Serum Block: 10% normal serum from the species the secondary antibody was made in; DAB for double staining; Organic Mounting Media; 0.1 M Tris HCl or PBS (See Reagent Preparation). RealBlue Peroxidase Substrate Kit is 50-100 times more sensitive than DAB and is non-carcinogenic. Strongly recommended for the detection of least-abundant markers. RealBlue forms a blue chromogenic product, providing excellent contrast with DAB and other substrates for multiple labeling experiments.
• Kit Type: Substrate Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

The application of antibodies and other proteins covalently coupled to horseradish peroxidase (HRP) in immunohistochemistry is well documented. In the presence of hydrogen peroxide (H2O2), HRP catalyzes the oxidation of TMB forming a blue chromogenic product visible by light microscopy. Researchers have shown that the reaction of TMB with HRP is a sensitive method for demonstration of retrograde tracing in neural tissue. Lu and Ho obtained excellent staining with TMB-molybdate in anterograde labeling of neural tissue at the ultrastructure level. When used in double-labeling for localization of innervations of feline cerebral arteries, TMB and DAB peroxidase substrates provided high resolution morphological results. Previous methods to stabilize the blue reaction color required multiple staining steps, but RealBlue provides permanent blue color when used as recommended. Like DAB, the RealBlue reaction product is insoluble in alcohols and Xylene. Endogenous peroxidase activity is eliminated by controlled oxidation prior to application of antibodies

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Real Blue Peroxidase Kit (KA0565)(1)

Publication using KA0565

• Bando Y, Sakashita H, Taira F et al. Origin and development of septoclasts in endochondral ossification of mice. Histochem Cell Biol 2018-02-20 [PMID: 29464321]

FAQs for Real Blue Peroxidase Kit (KA0565). (Showing 1 - 1 of 1 FAQ).

1. Does Novus have some multistaining kits for IHC?. Our customer needs to stain his IHC with with different reactivities in the same tissue and he is looking for kits with this function.
   • For the staining of multiple antigens, your customer may employ immunoenzymatic or immunofluorescence staining methods. For immunoenzymatic staining, we offer several chromogen, and you may suggest the following list of kits to the customer:, Reliable DAB Peroxidase Assay Kit 500 Slides (KA0562), RED Phosphatase Assay Kit 1000 Slides (KA0566), Real Blue Peroxidase Assay Kit 500 Slides (KA0565), ORANGE Peroxidase Assay Kit 1000 Slides (KA0563), BLUE Phosphatase Assay Kit 1000 Slides (KA0567). For immunofluorescence staining, we offer several types of secondary antibodies (conjugated to different immunofluorescent dyes) which may be used for labeling different antigens. The customer may chose the options from our secondary antibodies.