Raw 264.7 Nuclear Cell Lysate Summary
Nuclear extract cell lysate (non-denatured)
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2 mg/ml.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Raw 264.7
Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Publications for Raw 264.7 Lysate (L024V4) (0)
There are no publications for Raw 264.7 Lysate (L024V4).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Raw 264.7 Lysate (L024V4) (0)
There are no reviews for Raw 264.7 Lysate (L024V4).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Raw 264.7 Lysate (L024V4) (0)
Additional Raw 264.7 Products
Blogs on Raw 264.7