Rapid Tissue Preservation Solution

Immunohistochemistry-Paraffin: Rapid Tissue Preservation Solution [NBP3-12177] - Formalin fixed paraffin embedded human Melanoma stained with Melanoma antibody using Rapid Tissue Preservation Solution (NBP3-12177)

• Applications: IHC

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Preparation of Working Solutions
  Rapid Tissue Preservation Solution is a concentrated soution and requires dilution in reagent alcohol. Compatible alcohols include ethanol, methanol, isopropyl alcohol, or combinations thereof. Do not use alcohol that contains water. To prepare a working solution of Rapid Tissue Preservation Solution add 1 part of the concentration Rapid Tissue Preservation Solution to 9 parts of reagent alcohol.
  
  Protocol Recommendations for Single Slides
  1. After counterstaining, rinse slide with water.
  2. Wipe away excess water on both sides of the slide. Carefully wipe around tissue with an absorbent wipe.
  3. While holding the slide in a vertical position, drip Rapid-Histo-Sealer down the face of the slide to remove all water. Allow excess solution to run off the slide. Approximately 500 uL of Rapid Tissue Preservation Solution is sufficient to remove all water from a single slide.
  4. Carefully wipe excess Rapid Tissue Preservation Solution from around the tissue with an absorbent wipe.
  5. Lay slides flat and allow the sections to completely dry. Complete drying usually takes about 20 seconds
  6. Mount and coverslip slide using any resin-based mounting medium.

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Background

Rapid Tissue Preservation Solution is a preservation solution that acts to rapidly dehydrate tissues mounted on microscope slides in preparation for permanent mounting. This quick-acting dehydrating solution is ideal for rapid immunohistochemistry procedures. This solution seals the tissue to protect the tissues from drying artifacts to and improve tissue morphology. When applied to the stained section, specimens can be permanently mounted without chromogens fading. This product is designed for tissue specimens stained with peroxidase, alkaline phosphatase-based methods using a variety of chromogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.