QuikChIP Kit (ChIP)

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Chromatin Immunoprecipitation: Quick ChIP Kit [NBP2-29902] - Human MCF-7 cells were fixed for 10 minutes with 1% formaldehyde. The cells were lysed and the chromatin sonicated (200-1000 bp fragments). Chromatin was ...read more
Quick ChIP Kit [NBP2-29902]

Product Details

Summary
Reactivity Hu, Mu, BaSpecies Glossary
Applications ChIP, IP

Order Details

QuikChIP Kit (ChIP) Summary

Description
The QuikChIP(TM) Kit contains components for 25 ChIP assays optimized for use with transcriptionally active chromatin in adherent or suspension mammalian cells. The protocol is optimized for use with one 10 cm plate containing 5 x 10^6 cells per preparation. Each ChIP assay requires approximately 1 x 10^6 cells. Thus, a 10 cm plate (one preparation) of cells will provide DNA sufficient for five ChIP assays.

Features:
  • Quick and simple.
  • Optimized ready-to-use buffers and protocol are included.
  • Can be used for both histone and non-histone proteins.
  • Reagents for 25 ChIP assays and sheared DNA preparations.
  • Kit Type
    Kit (ChIP)

    Applications/Dilutions

    Dilutions
    • Chromatin Immunoprecipitation
    • Immunoprecipitation
    Application Notes
    Use in Immunoprecipitation reported in scientific literature (PMID 21957289).
    Publications
    Read Publications using
    NBP2-29902 in the following applications:

    • 36 publications
    • IP
      1 publication

    Reactivity Notes

    Mouse reactivity reported in scientific literature (PMID: 23709694). Bacteria reactivity reported in scientific literature (PMID: 24363348). Human reactivity reported in scientific literature (PMID: 21086175).

    Packaging, Storage & Formulations

    Storage
    Storage of components varies. See protocol for specific instructions.

    Kit Components

    Components
    1. 0.5M EDTA (250 uL)
    2. 100X PIC (2 x 0.75 mL)
    3. 100X PMSF (2 x 0.75 mL)
    4. 10X Glycine (25 mL)
    5. 10X PBS (55 mL)
    6. 1M NaHCO3 (1.25 mL)
    7. 1M Tris-HCl pH 6.5 (500 uL)
    8. 20% SDS (625 uL)
    9. 5M NaCl (500 uL)
    10. ChIP Dilution Buffer (50 mL)
    11. Proteinase K at 10 mg/mL (60 uL)
    12. RNase A at 10 mg/mL (60 uL)
    13. Salmon Sperm DNA/Protein A/G Agarose (2 x 1.75 mL)
    14. SDS Lysis Buffer (25 mL)
    15. Wash Buffer A (25 mL)
    16. Wash Buffer B (25 mL)
    17. Wash Buffer C (25 mL)
    18. Wash Buffer D (25 mL)

    Notes

    Additional Materials Required (not included in this kit):
    Reagents: Cells of interest, Antibody of interest, Appropriate antibody control, 37% Formaldehyde, 3M Sodium Acetate (pH 5.2), Phenol/chloroform (optional), 100% ethanol, 70% ethanol, Taq DNA polymerase, dNTP mixture, Cell culture media, ddH2O.
    Equipment: Agarose gel electrophoresis apparatus, Rotating platform, Microcentrifuge, Microcentrifuge tubes, Spectrophotometer, Variable volume pipettors, Pipette tips, Sonicator, Thermocycler, Thermocycling tubes, Cell Scraper, Aspirator.

    Background

    Chromatin Immunoprecipitation (ChIP) is a powerful method used to identify regions of the genome associated with specific proteins. These associations are crucial for vital cellular functions including gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. The ChIP assay has been used to study both histones and non-histone proteins, such as transcription factors, within the context of the cell. Transcription factors and other DNA binding proteins have a weaker affinity than histones, which generally are tightly associated within the chromatin complex. To avoid dissociation of non-histone proteins from the chromatin binding site, it is necessary to incorporate a cross-link step. QuikChIP(TM) Kit has been optimized for use with both histone and non-histone proteins. Using the QuikChIP(TM) Assay, cells are fixed with formaldehyde to cross-link nonhistone proteins to DNA. Alternatively, the cross-linking steps are not needed for histone proteins, which are tightly bound to DNA. The chromatin is released from the nuclei and sheared into 200-1000 bp fragments. The chromatin is then immunoprecipitated with specific antibodies. DNA sequences cross-linked to the protein of interest co-precipitate as part of the chromatin complex. The DNA/Chromatin/Antibody complex is then isolated using Protein G Agarose. After reverse cross-linking, the associated DNA is released from the complex and is ready for analysis.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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    Publications for QuikChIP Kit (NBP2-29902)(46)

    We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Insect.

    We have publications tested in 2 applications: ChIP, IP.


    Filter By Application
    ChIP
    (36)
    IP
    (1)
    All Applications
    Filter By Species
    Human
    (15)
    Mouse
    (6)
    Rat
    (1)
    Bacteria
    (1)
    Bovine
    (1)
    Insect
    (1)
    All Species
    Showing Publications 1 - 10 of 46. Show All 46 Publications.
    Publications using NBP2-29902 Applications Species
    Pal P, Roy S, Chowdhury A et al. Parkinson's Disease Associated 18 bp Promoter Variant of DJ-1 Alters Rest Binding and Regulates its Expression papers.ssrn.com Apr 4 2022 (ChIP) ChIP
    Zhou X, Li L, Guo X et al. HBXIP induces anoikis resistance by forming a reciprocal feedback loop with Nrf2 to maintain redox homeostasis and stabilize Prdx1 in breast cancer NPJ breast cancer Jan 13 2022 [PMID: 35027562]
    Pradeepa , Suresh V, Singh VK Et al. EVI1 promotes metastasis by downregulating TIMP2 in metastatic colon and breast cancer cells The international journal of biochemistry & cell biology Nov 18 2021 [PMID: 34800694] (ChIP) ChIP
    Wang M, Zheng Q, Zhao Z Et al. HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling Gastroenterology research and practice Oct 25 2021 [PMID: 34733326] (ChIP) ChIP
    Ma J, Xing B, Cao Y et al. Menin-regulated Pbk controls high fat diet-induced compensatory beta cell proliferation EMBO molecular medicine Apr 6 2021 [PMID: 33821572] (ChIP, Mouse) ChIP Mouse
    Nayak A, Roy AD, Rout N et al. HIF1 alpha-dependent upregulation of ATAD2 promotes proliferation and migration of stomach cancer cells in response to hypoxia Biochem. Biophys. Res. Commun. Jan 17 2020 [PMID: 31959473] (ChIP, Human) ChIP Human
    Wu Y, Doepner M, Hojnacki T et al. Disruption of the menin-MLL interaction triggers menin protein degradation via ubiquitin-proteasome pathway Am J Cancer Res Aug 1 2019 [PMID: 31497350] (ChIP, Human) ChIP Human
    Burlibaşa, L;Ionescu, AC;Dragusanu, DM; Histone hyperacetylation and DNA methylation interplay during murine spermatogenesis Zygote Aug 15 2019 [PMID: 31412961] (ChIP) ChIP
    Smith MP, Rana S, Ferguson J et al. A PAX3/BRN2 rheostat controls the dynamics of BRAF mediated MITF regulation in MITFhigh /AXLlow melanoma Pigment Cell Melanoma Res Oct 1 2018 [PMID: 30277012] (ChIP, Human) ChIP Human
    Leslie PL, Franklin DA, Liu Y et al. p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop Cell Rep Aug 7 2018 [PMID: 30089260] (ChIP, Human) ChIP Human
    Show All 46 Publications.

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