Quick Cell Proliferation Assay Kit (Colorimetric)

Product Discontinued

Quick Cell Proliferation Assay Kit (Colorimetric) Summary

• Description: Easy and sensitive WST based colorimetric assay to measure cell proliferation in response to growth factors, cytokines, mitogens and nutrients.
  
  Detection method: Absorbance at 420-480 nm.Applications: Measurement of cell cytotoxicity in response to drug or toxin treatmentQuantification of the cytotoxic effect of immune effector cells on target cellsAssessment of physiological mediators and antibodies that affect cell cytotoxicity
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65475)
• Kit Type: Assay Kit (Colorimetric)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

The assay is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. This method requires no washing, no harvesting, and no solubilization steps. The assay correlates well with the [3H]-thymidine incorporation assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.