pSIVA Apoptosis Detection Kit

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Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons: NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Addition of NGF ...read more
Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons. NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Correlation of ...read more
Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Quantitative analysis of pSIVA-IANBD™ fluorescence is rescued and NGF deprived neuronal cultures. ...read more
Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying photoreceptors in dystrophic rat neural retina. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, In vitro, In vivo, LIM
Conjugate
IANBD

Order Details

pSIVA Apoptosis Detection Kit Summary

Description
Polarity Sensitive Indicator of Viability (pSIVA(TM) is an Annexin based, polarity sensitive probe for the spatiotemporal or kinetic analysis of apoptosis and other forms of cell death. pSIVA(TM) binding is reversible, enabling researchers to detect transient phosphatidylserine (PS) exposure which is associated with normal physiological processes as well as with reversible or rescuable apoptosis cell death events. pSIVA(TM) is conjugated to IANBD, a polarity sensitive dye that fluoresces only when pSIVA(TM) is bound to the cell membrane. pSIVA's membrane-bound dependent fluorescence and reversible binding properties are a technological leap for detecting PS exposure and offer additional information on the apoptosis pathway and cell survival compared to Annexin V conjugates. Annexin V binding is nonreversible.
Kit Type
Kit

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • In vitro assay
  • In vivo assay
  • Live Imaging Microscopy
Application Notes
See attached PDF protocol for specific usage information. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24162663).
Reviewed Applications
Read 1 Review rated 5
using
NBP2-29382 in the following applications:

Publications
Read Publications using
NBP2-29382 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24999049). Human reactivity reported in scientific literature (PMID: 25692494).

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Propidium Iodide Staining Solution (500 uL)
  2. pSIVA-IANBD (200 uL)

Notes

pSIVA is protected under patent number: 8,541,549. This is the same kit which was sold as pSIVA(TM) - IANBD Apoptosis/Viability Microscopy Set [Catalog Number IMG-6701K] by Imgenex - now a part of Novus Biologicals, a Bio-Techne brand.

Alternate Names for pSIVA Apoptosis Detection Kit

  • apoptosis kinetics
  • apoptosis kit annexin v
  • apoptosis kit flow cytometry
  • apoptosis kit microscopy
  • apoptosis kit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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Publications for pSIVA Apoptosis Detection Kit (NBP2-29382)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: CellVia, Flow-CS, ICC/IF, In vitro, In vivo.


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(1)
Flow-CS
(2)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NBP2-29382 Applications Species
Li T, Yu D, Oak HC Et al. Phospholipid-flippase chaperone CDC50A is required for synapse maintenance by regulating phosphatidylserine exposure The EMBO journal Nov 2 2021 [PMID: 34585770]
Esposito NJ, Mazzoni F, Vargas JA, Finnemann SC Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3 Investigative ophthalmology & visual science Feb 1 2021 [PMID: 33538769] (Mouse) Mouse
Agier J, Brzezinska-Blaszczyk E, Witczak P et al. A Quenched Annexin V Fluorophore for the Real Time Fluorescence Imaging of Apoptotic Processes In Vitro and In Vivo Cellular Immunology Oct 28 2020 [PMID: 33158544] (In vivo, Mouse) In vivo Mouse
Zhang Y, Li H, Li X et al. TMEM16F Aggravates Neuronal Loss by Mediating Microglial Phagocytosis of Neurons in a Rat Experimental Cerebral Ischemia and Reperfusion Model Front Immunol Jul 7 2020 [PMID: 32733436]
Nardin C, Peres C, Mazzarda F et al. Photosensitizer Activation Drives Apoptosis by Interorganellar Ca2+ Transfer and Superoxide Production in Bystander Cancer Cells Cells 2019 Sep 29 [PMID: 31569545]
Kawasaki, K;Suzuki, Y;Yamamura, H;Imaizumi, Y; Rapid Na+ accumulation by a sustained action potential impairs mitochondria function and induces apoptosis in HEK293 cells expressing non-inactivating Na+ channels Biochem. Biophys. Res. Commun. May 21 2019 [PMID: 30952429] (ICC/IF, Human) ICC/IF Human
Almasieh M, Catrinescu MM, Binan L et al. Axonal Degeneration in Retinal Ganglion Cells is Associated with a Membrane Polarity-Sensitive Redox Process. J. Neurosci. Mar 8 2017 [PMID: 28275163] (ICC/IF, Rat) ICC/IF Rat
Tufail Y, Cook D, Fourgeaud L et al. Phosphatidylserine Exposure Controls Viral Innate Immune Responses by Microglia Neuron Jan 18 2017 [PMID: 28111081]
Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays. Rottgermann P, Dawson K, Radler J Microarrays (Basel) [PMID: 27600074] (CellVia, Human) CellVia Human
Sommer A, Kordowski F, Buch J et al. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nat Commun. May 10 2016 [PMID: 27161080] (ICC/IF, Human) ICC/IF Human
Show All 31 Publications.

Review for pSIVA Apoptosis Detection Kit (NBP2-29382) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP2-29382:
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Immunofluorescence
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence pSIVA Apoptosis Detection NBP2-29382
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5
reviewed by:
Silvia Finnemann
Immunofluorescence Rat 06/08/2016
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Summary

ApplicationImmunofluorescence
Sample Testeddystrophic (RCS) rat neural retina
SpeciesRat
Lot060515.1

Product General Protocols

View specific protocols for pSIVA Apoptosis Detection Kit (NBP2-29382): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for pSIVA Apoptosis Detection Kit (NBP2-29382). (Showing 1 - 2 of 2 FAQs).

  1. Our end user is working on tissues (epithelium of imaginal disk from drosophila leg). The tissues are kept growing on a culture medium and they manage to follow apoptosis properly with orange acrylin but not with annexin V because of background. Did you ever test any of your pSIVA apoptosis kits (NBP2-29382, NBP2-29611) on tissue culture instead of cell culture ? Is there a chance the proble will work on such an application ? Would you be able to provide a sample for this application ? As for the other samples, I'm still waiting on customer feedback and I'll keep you posted.
    • Yes, pSIVA has been used in tissue applications as denoted on the publication link. Specifically, I would encourage the research to look at the following publications from the link: 1. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Ruggiero L, MP Connor, J Chen, R Langen, SC Finnemann. PNAS 109:8145-4148 (2012). Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). > The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. 2. Diurnal photoreceptor outer segment shedding: contributions of the RPE. Finnemann SC, L Ruggiero, MP Connor. Invest Opthalmol Vis Sci 53:4327 (2012). Live Imaging: mouse retina. pSIVA was used quantify the surface exposure of phosphotidylserine on the distal rod photoreceptor outer segments. 3. Characterization of cell cycle modifications induced by electric pulses in human corneal endothelium. Thi MH, Z He, N Campolmi, S Piselli, P Gain, M Peoc'H, JM Dumolllard, S Acquart, O Garraud, G Thuret. Acta Opthalmologica 90:s249 (2012). Live tissue imaging (human cornea organ cultures): pSIVA-IANBD was used to assess cell death following gene electrotransfer into corneal endothelial cells. > 3. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processess and degeneration. Kim YE, J Chen, JR Chan, R Langen. Nature Methods 7:67-73 (2010a). Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (COS-7 cells), Fig 3 (rat neuronal degeneration), Fig 4 (rat axonal degeneration), Fig 5 (rescue of rat neuronal degeneration as visualized by pSIVA. (this was also used in a rat nerve injury tissue model) Please watch the pSIVA webinar on our website for additional information about pSIVA. The webinar is referred to March 25, 2014: Real-Time Tracking of Cell Death Webinar. We would certainly be pleased to offer any of your end user research customers a sample who is willing to provide a short explanation about what they are planning to do, their timeframe, and provide feedback about how it worked for them. For any samples, please specify if you
  2. How many tests can you perform with this kit?
    • 100 tests for the full sized kit and 25 tests for the 1 sample sized kit.

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Recent Reviews

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Silvia Finnemann
06/08/2016
Application: Immunofluorescence
Species: Rat