Protocadherin gamma A8 Antibody (RB31662)

Western Blot: Protocadherin gamma A8 Antibody [NBP2-30028] - PCDHGA8 Antibody (C-term) (NBP2-30028) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow).

Immunocytochemistry/Immunofluorescence: Protocadherin gamma A8 Antibody [NBP2-30028] - Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term)(NBP2-30028) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry-Paraffin: Protocadherin gamma A8 Antibody [NBP2-30028] - PCDHGA8 Antibody (C-term) (NBP2-30028)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Discontinued

Protocadherin gamma A8 Antibody (RB31662) Summary

• Immunogen: This PCDHGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 781-808 amino acids from the C-terminal region of human PCDHGA8.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCDHGA8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Western Blot 1:1000
• Theoretical MW: 101.48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.32 mg/ml
• Purity: Protein A purified

Alternate Names for Protocadherin gamma A8 Antibody (RB31662)

• KIAA0327PCDH-GAMMA-A8
• PCDH-gamma-A8
• protocadherin gamma subfamily A, 8
• protocadherin gamma-A8

Background

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHGA8
• Uniprot:
  • Q9Y5G5(Human)