Recombinant Human Proteinase K Protein

Product Discontinued

Recombinant Human Proteinase K Protein Summary

• Description: Recombinant protein for Human Proteinase K
  
  Source:Tritirachium album
  Amino Acid Sequence:(P06873)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab286002)
• Source: Fungus
• Protein/Peptide Type: Recombinant Protein
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: >30 units/mg
• Theoretical MW: 28.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 50 mM Tris-HCl (pH 8.0), 2 mM Calcium acetate
• Concentration: Lyoph
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with deionized H2O.

Alternate Names for Recombinant Human Proteinase K Protein

• Endopeptidase K
• PROK
• Proteinase K
• Tritirachium alkaline proteinase

Background

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.