Recombinant Protein L Type 1 His - (N-Term)Protein

Product Discontinued

Recombinant Protein L Type 1 His - (N-Term)Protein Summary

• Description: Amino acid 98-462 of the Peptostreptococcus Magnus. Protein L Contains 5 kappa-binding domain with 6x Histag on N-terminus. Gene Bank Accession Number: AAA25612
• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Binding Capacity: Under optimal conditions, 1 mg protein L will bind approximately 5 mg human IgG. Optimal binding of Protein L to antibodies is at pH 7.5.
• Theoretical MW: 43.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized with no additives.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to 5 mg/ml.

Background

Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. 's Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Entrez:
  • AAA25612.1(Bacterial)
• Uniprot:
  • Q51912(Bacterial)