Recombinant Protein L Type 1 Protein

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Product Details

Summary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Protein L Type 1 Protein Summary

Description
Recombinant protein for Human Protein L Type 1

Source:E. coli
Amino Acid Sequence:(CAA25612)

Preparation
Method
Determined to be >98% pure by SDS-PAGE
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Application Notes
Binding Capacity: Under optimal conditions, 1 mg protein L will bind approximately 5 mg human IgG. Optimal binding of Protein L to antibodies is at pH 7.5.
Theoretical MW
43.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized with no additives
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitution in H2O to a concentration of 5 mg/ml gives a clear solution.

Background

Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. 's Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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