Recombinant Bacteria Protein A Protein [Biotin]

Product Discontinued

Recombinant Bacteria Protein A Protein [Biotin] Summary

• Description: Recombinant protein for Bacteria Protein A
  
  

  Source:E. coli
  
  

  Conjugated with Biotin

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Binding Capacity: Under optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
• Theoretical MW: 59.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized with no additives.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to 5 mg/ml.

Alternate Names for Recombinant Bacteria Protein A Protein [Biotin]

• Immunoglobulin G binding protein A
• PROA
• Protein A
• Protein-A
• SPA
• Staphylococcal protein A

Background

Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Protein A Recombinant Protein (NBP1-99211). (Showing 1 - 1 of 1 FAQ).

1. During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!
   • We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.