Protein A Native Protein

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Immunoprecipitation: Protein A Protein [Sepharose] [NBP1-97288] - Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, IP
Conjugate
Sepharose
Concentration
6.0 mg/ml

Order Details

Protein A Native Protein Summary

Description
Native Protein A protein.

Source:

Preparation
Method
rotein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
Protein/Peptide Type
Native Protein

Applications/Dilutions

Application Notes
This product is suitable for use at 20 uL per immunoprecipitation reaction.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
20% (v/v) Ethanol
Preservative
No Preservative
Concentration
6.0 mg/ml

Alternate Names for Protein A Native Protein

  • Immunoglobulin G binding protein A
  • SPA
  • Staphylococcal protein A

Background

Sepharose Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureaus. When evenly dispersed approximately 10 mL of suspension is equal to 5.0 cc of settled beads of Sepharose 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 um when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for Protein A Protein (NBP1-97288). (Showing 1 - 1 of 1 FAQ).

  1. During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!
    • We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

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