Protein A Native Protein

N/A: Protein A Native Protein [NBP1-97240] - Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain.

N/A: Protein A Native Protein [NBP1-97240]

• Reactivity: Ba
• Applications: WB, IP
• Conjugate: Sepharose

Protein A Native Protein Summary

• Description: Native Protein A (Accession #: P38507))
  
  Store SEPHAROSE PROTEIN A at 4C prior to opening. DO NOT FREEZE.
• Preparation Method: Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose(TM) Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
• Protein/Peptide Type: Native Protein
• Gene: SAOUHSC_00069
• Purity: N/A

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 uL per immunoprecipitation reaction.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20% (v/v) Ethanol
• Preservative: No Preservative
• Purity: N/A

Alternate Names for Protein A Native Protein

• Immunoglobulin G binding protein A
• PROA
• Protein A
• Protein-A
• SPA
• Staphylococcal protein A

Background

Sepharose Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 um when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Protein A Native Protein (NBP1-97240)(1)

Publication using NBP1-97240

• Luo W, Chen I, Chen Y et al. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia Oncotarget. 2016-02-09 [PMID: 26837221] (WB)
  
  Details:
  The mechanism for feedback inhibition of hypoxia-inducible factors during prolonged hypoxia is clarified through studying the interaction of PRDX2 and PRDX4 with HIF-1 alpha and HIF-2 alpha.

FAQs for Protein A Native Protein (NBP1-97240). (Showing 1 - 1 of 1 FAQ).

1. During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!
   • We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

Bioinformatics

• Gene Symbol: SAOUHSC_00069
• Uniprot:
  • P38507(Bacteria)