Recombinant Protein A/G Protein

• Applications: Func, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Protein A/G Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 430 amino acids corresponding to Protein A/G Source: E. coli

  Amino Acid Sequence: CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE

• Specificity: Recombinant Protein A/G binds to various human, mouse and rat IgG subclasses (e.g. human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c) and to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.
• Details of Functionality: Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the Protein A/G to ensure the maximum specific IgG binding. Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. It binds to various human, mouse and rat IgG subclasses and to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Protein A/G as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Functional
  • SDS-Page
• Theoretical MW: 47.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from additive free solution.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Dissolve in distilled water or saline.

Background

Protein A/G consists of 7 IgG-binding domains EDABC-C1C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.