Prostaglandin F2 alpha ELISA Kit (Colorimetric)

ELISA: Non-species specific Prostaglandin F2 alpha ELISA Kit (Colorimetric) [NBP2-62167] - A typical standard curve for Prostaglandin F2 alpha ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: Culture supernatants, plasma, serum, saliva, urine, cell lysate, whole blood
• Standard Curve Range: 3.05 - 50,000 pg/ml
• Sensitivity: 6.71 pg/ml

Prostaglandin F2 alpha ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 3.05 - 50,000 pg/ml
• Sensitivity: 6.71 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 9.7% Medium Concentration %CV - 5.5% High Concentration %CV - 3.1%
• Intra-Assay: Low Concentration %CV - 13.1% Medium Concentration %CV - 6.8% High Concentration %CV - 4.9%
• Spike Recovery: Tissue Culture Media - 100%
  Human Saliva - 103.5%
  Human Urine - 107.0%
  Porcine and Human Serum - 100.3%
  Porcine Plasma - 96.9%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Prostaglandin F2 alpha ELISA Kit (Colorimetric)

• PG F2 alpha
• PGF 2 alpha
• PGF2 alpha
• PGF2alpha
• Prostaglandin F2alpha
• ProstaglandinF2 alpha

Background

Prostaglandin F2alpha (PGF2alpha) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2alpha is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2alpha is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2alpha is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2alpha have also been associated with preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Prostaglandin F2 alpha ELISA Kit (NBP2-62167)(1)

Publication using NBP2-62167

• Powers Aa, Jones Ke, Eisenberg Sh Et Al. Experimental respiratory exposure to putative Gulf War toxins promotes persistent alveolar macrophage recruitment and pulmonary inflammation Life sciences 2021-07-19 [PMID: 34293400] (ELISA)

FAQs for Prostaglandin F2 alpha ELISA Kit (NBP2-62167). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.