Prostaglandin F2 alpha ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
1.95 to 2000 pg/mL |
| Sensitivity |
0.98 pg/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 9.99% |
| Intra-Assay |
CV% < 6.21% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
PGF2-alpha High Sensitivity ELISA Kit is a competitive immunoassay for the quantitative determination of Prostaglandin F2-alpha in milk, culture supernates, saliva, urine, serum and plasma. |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- 0.1M HCl
- Acetic Anhydride
- Assay Buffer 2
- cAMP Antibody
- cAMP Conjugate
- cyclic AMP Standard
- Goat anti-Rabbit IgGMicrotiter Plate
- Neutralizing Reagent
- Plate Sealer
- pNpp Substrate
- Stop Solution
- Triethylamine
- Wash BufferConcentrate
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Prostaglandin F2 alpha ELISA Kit (Colorimetric)
Background
Prostaglandin F2alpha (PGF2alpha) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2alpha is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2alpha is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2alpha is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2alpha have also been associated with preeclampsia.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Prostaglandin F2 alpha ELISA Kit (KA0327). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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