Propidium Iodide is excluded by viable cells but can penetrate cell membranes of dying or dead cells. Dead cells will take up PI and fluoresce brightly in FL2 off of the blue laser on a standard flow cytometer. PI also may be excited by the UV or Green/Yellow lasers on more advanced flow cytometers and may, therefore, not be optimal for certain multicolor panels. It emission maxima is at 617nm.
Fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.
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