Progesterone/OHP ELISA Kit (Colorimetric) Summary
| Description |
17-OH-Progesterone ELISA Kit is an enzyme immunoassay for the quantitative measurement of active free 17-OH-Progesterone in saliva. |
| Standard Curve Range |
10 to 1000 pg/mL |
| Sensitivity |
n/a |
| Assay Type |
Quantification |
| Inter-Assay |
CV% < 5.33% |
| Intra-Assay |
CV% < 5.23% |
| Spike Recovery |
100.98% |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
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Components
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- Control 1 & Control 2: contain 0.03% Proclin 300, 0.015% BND and 0.010%MIT as preservative .For control values and ranges please refer to vial labelor QC-Datasheet. Ready to use.
- Cortisol Standard A, 0 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard B, 10 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard C, 50 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard D, 250 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard E, 500 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard F, 1000 pg/ml, contain 0.03% Proclin 300, 0.015% BNDand 0.010% MIT as preservative.
- Microtiterwells: coated with a anti-17a-OHP antibody (polyclonal) 96 well (12x8 strips)Enzyme conjugate: 17a-OHP conjugated to horseradish peroxidase, contain0.03 % Proclin 300, 0.015% BND and 0.010% MIT as preservative. Ready touse.
- Microtiterwells: coated with a anti-17a-OHP antibody (polyclonal)
- Stopping Solution: containing 0.5 M H2SO4. Avoid contact with the stopsolution. It may cause skin irritations and burns.
- Substrate Solution: Tetramethylbenzidine (TMB)
- Wash Solution (40X concentrated)
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Progesterone/OHP ELISA Kit (KA1896). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
Additional Progesterone/OHP Products
Blogs on Progesterone/OHP
![ELISA: Progesterone/OHP ELISA Kit (Colorimetric) [KA1896] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Progesterone-OHP-ELISA-Kit-Colorimetric-ELISA-KA1896-img0001.jpg "ELISA: Progesterone/OHP ELISA Kit (Colorimetric) [KA1896] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
