Progesterone/OHP ELISA Kit (Colorimetric) Summary
| Description |
17-OH-Progesterone ELISA Kit is an enzyme immunoassay for the quantitative measurement of active free 17-OH-Progesterone in saliva. |
| Standard Curve Range |
10 to 1000 pg/mL |
| Sensitivity |
n/a |
| Assay Type |
Quantification |
| Inter-Assay |
CV% < 5.33% |
| Intra-Assay |
CV% < 5.23% |
| Spike Recovery |
100.98% |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
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Components
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- Control 1 & Control 2: contain 0.03% Proclin 300, 0.015% BND and 0.010%MIT as preservative .For control values and ranges please refer to vial labelor QC-Datasheet. Ready to use.
- Cortisol Standard A, 0 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard B, 10 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard C, 50 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard D, 250 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard E, 500 pg/ml, contain 0.03% Proclin 300, 0.015% BND and0.010% MIT as preservative.
- Cortisol Standard F, 1000 pg/ml, contain 0.03% Proclin 300, 0.015% BNDand 0.010% MIT as preservative.
- Microtiterwells: coated with a anti-17a-OHP antibody (polyclonal) 96 well (12x8 strips)Enzyme conjugate: 17a-OHP conjugated to horseradish peroxidase, contain0.03 % Proclin 300, 0.015% BND and 0.010% MIT as preservative. Ready touse.
- Microtiterwells: coated with a anti-17a-OHP antibody (polyclonal)
- Stopping Solution: containing 0.5 M H2SO4. Avoid contact with the stopsolution. It may cause skin irritations and burns.
- Substrate Solution: Tetramethylbenzidine (TMB)
- Wash Solution (40X concentrated)
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Progesterone/OHP ELISA Kit (KA1896). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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