Recombinant T. gondii Profilin Protein

• Reactivity: Pz
• Applications: PAGE
• Concentration: LYOPH

Recombinant T. gondii Profilin Protein Summary

• Description: Recombinant profilin (Toxoplasma gondii) (aa 1-163).
  
  Source: E. coli

  Uniprot ID: Q58NA1

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: <0.01EU/ug purified protein (LAL test; Lonza).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95% pure by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)

Alternate Names for Recombinant T. gondii Profilin Protein

• PRF

Background

TLR11, an innate sensor for profilin (Toxoplasma gondii), is an intracellular receptor that resides in the endoplasmic reticulum. The 12 membrane-spanning endoplasmic reticulum-resident protein UNC93B1 interacts directly with TLR11 and regulates the activation of dendritic cells in response to profilin (Toxoplasma gondii) and parasitic infection in vivo. TLR12, a previously uncharacterized TLR, also recognizes profilin from Toxoplasma gondii. TLR12 is sufficient for recognition of profilin by plasmacytoid dendritic cells (pDCs), whereas TLR11 and TLR12 are both required in macrophages and conventional DCs. In contrast to TLR11, TLR12-deficient mice succumb rapidly to T. gondii infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q58NA1(T. gondii)