Pretreatment I Buffer

• Reactivity: Hu
• Applications: Simple Plex

Pretreatment I Buffer Summary

• Additional Information: Pretreatment I buffer is intended for use with the Simple Plex Protein A Cartridge Kit.
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • Simple Plex Automated Immunoassay

Packaging, Storage & Formulations

• Storage: Store Pretreatment K buffer at 2-8 °C. Discard remaining buffer after opening.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pretreatment I Buffer

• Immunoglobulin G binding protein A
• PROA
• Protein A
• Protein-A
• SPA
• Staphylococcal protein A

Background

Protein A is a Staphylococcus aureus cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially those of the IgG class. Because of this property, it is often utilized for microscopic visualization of molecules and to purify antisera. Protein A has also been used for the treatment of proteinuria in nephrotic syndrome, and severe autoimmune diseases such as rheumatoid arthritis, coeliac disease, and systemic lupus erythematosis.

FAQs for Protein A (897014). (Showing 1 - 1 of 1 FAQ).

1. During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!
   • We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.