Pregnenolone ELISA Kit (Colorimetric)

ELISA: Pregnenolone ELISA Kit (Colorimetric) [NBP2-68102] - Samples were spiked with high concentrations of Pregnenolone and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Pregnenolone ELISA Kit (Colorimetric) [NBP2-68102] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Pregnenolone ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Pregnenolone in samples. No significant cross-reactivity or interference between Pregnenolone and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: %CV < 5.43
• Intra-Assay: %CV < 4.6
• Spike Recovery: 85 - 108%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Pregnenolone ELISA Kit (NBP2-68102)(4)

Publications using NBP2-68102

• Miriam Ferrer, Nicholas Mourikis, Emma E. Davidson, Sam O. Kleeman, Marta Zaccaria, Jill Habel, Rachel Rubino, Qing Gao, Thomas R. Flint, Lisa Young, Claire M. Connell, Michael J. Lukey, Marcus D. Goncalves, Eileen P. White, Ashok R. Venkitaraman, Tobias Janowitz Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia Cell metabolism 2024-04-23 [PMID: 37311455]
• Lu J, Shang X, Yao B et al. The role of CYP1A1/2 in cholesterol ester accumulation provides a new perspective for the treatment of hypercholesterolemia Acta Pharmaceutica Sinica B 2022-08-01 [PMID: 36873188]
• Ferrer M, Mourikis N, Davidson EE et al. Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia bioRxiv : the preprint server for biology 2023-02-18 [PMID: 36824830] (ELISA, Mouse)
• Manna PR, Reddy AP, Pradeepkiran JA et al. Regulation of retinoid mediated StAR transcription and steroidogenesis in hippocampal neuronal cells: Implications for StAR in protecting Alzheimer's disease Biochimica et biophysica acta. Molecular basis of disease 2022-11-07 [PMID: 36356843] (ELISA)

FAQs for Pregnenolone ELISA Kit (NBP2-68102). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.