Pregnenolone ELISA Kit (Colorimetric)

ELISA: Pregnenolone ELISA Kit (Colorimetric) [NBP2-68102] - Samples were spiked with high concentrations of Pregnenolone and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Pregnenolone ELISA Kit (Colorimetric) [NBP2-68102] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Pregnenolone ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Pregnenolone in samples. No significant cross-reactivity or interference between Pregnenolone and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: %CV < 5.43
• Intra-Assay: %CV < 4.6
• Spike Recovery: 85 - 108%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Pregnenolone ELISA Kit (NBP2-68102)(4)

Publications using NBP2-68102

• Miriam Ferrer, Nicholas Mourikis, Emma E. Davidson, Sam O. Kleeman, Marta Zaccaria, Jill Habel, Rachel Rubino, Qing Gao, Thomas R. Flint, Lisa Young, Claire M. Connell, Michael J. Lukey, Marcus D. Goncalves, Eileen P. White, Ashok R. Venkitaraman, Tobias Janowitz Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia Cell metabolism 2024-04-23 [PMID: 37311455]
• Lu J, Shang X, Yao B et al. The role of CYP1A1/2 in cholesterol ester accumulation provides a new perspective for the treatment of hypercholesterolemia Acta Pharmaceutica Sinica B 2022-08-01 [PMID: 36873188]
• Ferrer M, Mourikis N, Davidson EE et al. Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia bioRxiv : the preprint server for biology 2023-02-18 [PMID: 36824830] (ELISA, Mouse)
• Manna PR, Reddy AP, Pradeepkiran JA et al. Regulation of retinoid mediated StAR transcription and steroidogenesis in hippocampal neuronal cells: Implications for StAR in protecting Alzheimer's disease Biochimica et biophysica acta. Molecular basis of disease 2022-11-07 [PMID: 36356843] (ELISA)

FAQs for Pregnenolone ELISA Kit (NBP2-68102). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.