PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit (Colorimetric) Summary
| Description |
PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR-alpha, delta, gamma transcription factor DNA binding activity in nuclear extracts and whole cell lysates. |
| Assay Type |
Colorimetric |
| Sample Volume |
10 uL/well. All samples should be assayed in duplicate at minimum. |
| Kit Type |
Assay Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- 96-Well Cover Sheet
- Polysorbate 20
- Transcription Factor Antibody Binding Buffer (10X)
- Transcription Factor Binding Assay Buffer (4X)
- Transcription Factor Complete PPARalpha Positive Control
- Transcription Factor Complete PPARalpha Primary Antibody
- Transcription Factor Complete PPARdelta Positive Control
- Transcription Factor Complete PPARdelta Primary Antibody
- Transcription Factor Complete PPARgamma Positive Control
- Transcription Factor Complete PPARgamma Primary Antibody
- Transcription Factor Developing Solution
- Transcription Factor Goat Anti-Rabbit HRP Conjugate
- Transcription Factor PPAR 96-Well Strip Plate
- Transcription Factor PPAR Specific Competitor dsDNA
- Transcription Factor Reagent A
- Transcription Factor Stop Solution
- Wash Buffer Concentrate (400X)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR-alpha, delta, gamma transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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![PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit (Colorimetric) [KA1357] - Increasing amounts of positive control (total lysate) are assayed for PPARA, PPARD, and PPARG DNA-binding activity.](http://images.novusbio.com/fullsize/PPARA-PPARD-PPARG-Complete-Transcription-Factor-Assay-Kit-Colorimetric-KA1357-img0001.jpg "PPARA/PPARD/PPARG Complete Transcription Factor Assay Kit (Colorimetric) [KA1357] - Increasing amounts of positive control (total lysate) are assayed for PPARA, PPARD, and PPARG DNA-binding activity.")
