Porcine IgG, Fc Native Protein

SDS-Page: Porcine IgG, Fc Native Protein [NBP1-97004] - SDS-PAGE of Swine IgG F(c). Lane 1: Non-reduced Swine IgG F(c). Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Swine IgG F(c). Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 50, 25, 12kDa, Reduced at 25, 12 kDa/Non-reduced at 25, 12 kDa, Reduced at 25, 12 kDa.

Product Discontinued

Porcine IgG, Fc Native Protein Summary

• Description: Native Porcine Porcine IgG, Fc
  
  Source: Normal serum (Accession #:AAB22049.1)
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine Serum, anti-Swine IgG and anti-Swine IgG F(c). No reaction was observed against anti-Swine IgG F(ab')2 or anti-Papain. By SDS-PAGE this material may appear as 50, 25 and 12 kDa bands under non-reducing conditions and predominately 25 and 12 kDa bands under reducing conditions. This is normal for papain digested Swine IgG. These bands consist of determinants derived entirely from F(c) portions of Swine IgG.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • SDS-Page
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Multi-step

Notes

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Porcine IgG, Fc Native Protein (NBP1-97004)(1)

Publication using NBP1-97004

• Niedermair T, Schirner S, Straub RH, GrAssel S Absence of alpha-calcitonin gene-related peptide modulates bone remodeling properties of murine osteoblasts and osteoclasts in an age-dependent way Mech. Ageing Dev. 2020-05-21 [PMID: 32446790] (ICC/IF, Mouse)