Porcine IgG, Fab Native Protein

• Reactivity: Po

Porcine IgG, Fab Native Protein Summary

• Description: Native Porcine Porcine IgG, Fab
  
  Source: Normal serum (Accession #:6912366)
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine Serum, anti-Swine IgG and anti-Swine IgG F(ab')2. No reaction was observed against anti-Swine IgG F(c) or anti- Papain.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Application Notes: This product has been tested by SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Background

Swine IgG Fab Fragment - secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.