Polyethylene Glycol Antibody Pair

Product Discontinued

Polyethylene Glycol Antibody Pair Summary

• Description: Quality control test: Standard curve using LipoDox as an analyte. This matched antibody pair set binds to the repeating subunits of the polyethylene glycol polymer and can be employed to detect and quantify PEGylated compounds.
  
  
• Capture antibody: mouse monoclonal anti-PEG IgM (100 ug). Stored in PBS buffer with 0.02% Sodium Azide.
• Detection antibody: biotinylated mouse monoclonal anti-PEG IgG1 (100 ug). Stored in PBS buffer with 0.02% Sodium Azide and 50% Glycerol.
• Clonality: Monoclonal

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 0.1 ng/ml - 30 ng/ml

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Polyethylene Glycol Antibody Pair

• 1,2-Dihydroxyethane
• 1,2-ethanediol
• 107-21-1
• 2-hydroxyethanol
• Ethane-1,2-diol
• Glycol alcohol
• glycol
• monoethylene glycol

Background

Polyethylene glycol (PEG) is a family of long chain polymers attached to a glycerine backbone. PEG is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer. Polyethylene glycol is commonly used to fuse B-cells with myeloma cells in monoclonal antibody production. Anti-PEG antibodies can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion. In addition, PEG antibodies can be used for the quality control of pegylated molecules in ELISA, WB and flow cytometery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Publications for Polyethylene Glycol Antibody Pair (AP0002)(7)

Publications using AP0002

• Moyers JS, Volk CB, Cao JXC et al. Internalization and localization of basal insulin peglispro in cells. Mol Cell Endocrinol 2017-05-30 [PMID: 28576743]
• Sherman MR, Williams LD, Sobczyk MA et al. Role of the Methoxy Group in Immune Responses to mPEG-Protein Conjugates. Bioconjug Chem. 2012-03-07 [PMID: 22332808]
• Su YC, Chen BM, Chuang KH et al. Sensitive Quantification of PEGylated Compounds by Second-Generation Anti-Poly(ethylene glycol) Monoclonal Antibodies. Bioconjug Chem. 2010-06-10 [PMID: 20536171]
• Chuang KH, Wang HE, Chen FM et al. Endocytosis of PEGylated Agents Enhances Cancer Imaging and Anticancer Efficacy. Mol Cancer Ther. 2010-05-25 [PMID: 20501805]
• Chuang KH, Wang HE, Cheng TC et al. Development of a Universal Anti-Polyethylene Glycol Reporter Gene for Noninvasive Imaging of PEGylated Probes. J Nucl Med. 2010-05-19 [PMID: 20484433]
• Chuang KH, Tzou SC, Cheng TC et al. Measurement of poly(ethylene glycol) by cell-based anti-poly(ethylene glycol) ELISA. Anal Chem. 2010-03-15 [PMID: 20178318]
• Cheng TL, Cheng CM, Chen BM et al. Monoclonal antibody-based quantitation of poly(ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjug Chem. 2005-09-01 [PMID: 16173802]