PNAS-127 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC206178 and based on accession number NM_032490. The protein contains a C-terminal DDK tag.
Homo sapiens chromosome 14 open reading frame 142 (C14orf142), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Lysate Details for PNAS-127
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for PNAS-127 Overexpression Lysate (Native)
- uncharacterized protein C14orf142
- chromosome 14 open reading frame 142
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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