Mouse Placenta Whole Tissue Lysate (Adult Whole Normal)

• Reactivity: Mu
• Applications: WB
• Concentration: 5.0 mg/ml

Mouse Placenta Whole Tissue Lysate (Adult Whole Normal) Summary

• Description: We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
• Specificity: Total Protein - Mouse Normal Tissue: Placenta

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Reproductive
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Placenta Lysate (NB820-59666). (Showing 1 - 2 of 2 FAQ).

1. Which mouse strain is used for the current lot?
   • The strain is Balb/c.
2. I have query regarding the product Mouse Placenta Whole Tissue Lysate (Adult Normal) 1 mg (Cat no. NB820-59666). Is this product can be used for In Vivo studies? Also what can I use (which buffer) to dilute the lysate.
   • Our Mouse Placenta Whole Tissue Lysate (Adult Normal) with catalogue number NB820-59666 is designed for Western blotting and immunoprecipitation, and I would not recommend that you use it for in vivo work. The precise manufacturing process is proprietary, however the lysate is supplied in HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors. To further dilute the lysate I suggest using this buffer. The recommended storage is -80C and freeze-thaw cycles should be avoided.