| Applications | WB |
| Clone | 450401 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 647 |
| Immunogen | E. coli-derived recombinant human PKCι Ile455-Val596 Accession # P41743 |
| Specificity | Detects endogenous human and mouse PKC iota / lambda in Western blots. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity Statement | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied |
| Buffer | Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide |
Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their cofactor requirements: conventional PKC isoforms ( alpha , beta Ι, beta II, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon , eta , and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota / lambda , and zeta ), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC iota is highly expressed in brain and lung but is also expressed at lower levels in many tissues including pancreatic islets. It is the catalytic component of the Par3-Par6-atypical PKC complex, a key regulator of the formation and maintenance of epithelial cell polarity, adherens junctions, and tight junctions. PKC iota has also been shown to be critical for Ras-mediated transformation, invasion, and anchorage‑independent growth of intestinal epithelial cells. The rodent homolog is termed PKC lambda and is 95% homologous to PKC iota .
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