PIR-A/B Antibody (10-1-PIR) [Allophycocyanin]

Product Discontinued

PIR-A/B Antibody (10-1-PIR) [Allophycocyanin] Summary

• Immunogen: The immunogen for this antibody is PIR-A/B.
• Specificity: Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: This 10-1-PIR antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for PIR-A/B Antibody (10-1-PIR) [Allophycocyanin]

• PIR-A/B

Background

This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating (PIR)-A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs (ITAMs) which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs (ITIMs); therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.