PiColorlock™ ALS Assay Kit (Colorimetric)

Product Discontinued

PiColorlock™ ALS Assay Kit (Colorimetric) Summary

• Description: PiColorLock has been developed for measuring the activity of any Pi-generating enzyme. It is a superior phosphate detection reagent, with high stability of the dye-phosphate complex (unlike others that are prone to precipitation). It provides an alternative to hazardous radioactive methods and other less sensitive colorimetric assays. In our Phosphate Detection Systems, PiColorLock reagent is supplied with an Accelerator to overcome and with a Stabiliser to prevent high background signals with acid-labile substrates, as well as a Pi standard to prepare a standard curve.
  
  Features:
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of Pi-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.
  • Colorimetric, non-radioactive assay.
  
  There are two types of PiColorLock (and hence Phosphate Detection System) available:
  
  
  • PiColorLock ALS - standard sensitivity (developed for microplates and cuvettes)
  
  
  • PiColorLock Gold - high sensitivity (developed for microplates)
  125.0 ml: Enough for 625 or 1560 assays is provided, depending on plate type. This kit is enough for 325 assays using 200ul PiColorLock Gold for 50ul samples (using 96-well plates) or for 1560 assays using 80ul PiColorLock Gold for 20ul samples (using 384-well plates).
• Specificity: PiColorlock ALS Kit (625 Tests)
  
  Phosphatases, ATPases and several other enzymes catalyse reactions in which inorganic phosphate (Pi) is released from a substrate. PiColorLock ALS has been developed formeasuring the activity of any Pi-generating enzyme. The reagent is formulated to give sensitive detection of Pi. It provides an alternative to hazardous radioactive methods and other less sensitive colorimetric assays.
  
  The measurement of Pi is based on the change in absorbance of malachite green in the presence of molybdate. Unlike other malachite dye formulations, PiColorLock ALS gives a completely stable end-point signal and is not prone to precipitation. Moreover, a proprietary stabilizer ensures that PiColorLock ALS can be used even with acid labile substrates.
  
  Features that make the PiColorlock ALS Kit such a powerful tool:
  
  Colorimetric, non-radioactive assay.
  Unparalleled stability of Pi-dye complexes.
  Stable reagent formulation - long shelf life.
  Reagent is compatible with almost any assay buffer.
  No inhibition of color development by high concentrations of protein.
  Special additive ensures low backgrounds with acid-labile substrates.
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

Publications

Read Publication using 302-0125.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Colorlock 625/1560 assays reagent is supplied with a phosphate standard solution

Notes

PiColorlock® is a registered trademark of Expedeon Inc and distributed by Novus Biologicals.

Background

Colorimetric assays are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate. To resolve this issue scientists developed PiColorLock - a superior phosphate detection reagent which ensures high stability of the colored dye phosphate complexes, thus leading to outstanding assay performance. Kit sensitivity available: PiColorlock ALS - Standard Sensitivity PiColorlock Gold - High Sensitivity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for PiColorlock (TM) ALS Kit (302-0125)(1)

Publication using 302-0125

• Low HH, Lowe J. A bacterial dynamin-like protein. Nature 2006-12-07 [PMID: 17122778]