PiBind Resin

PiBind Resin [501-0015]

PiBind Resin Summary

• Description: Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds.
  
  PiBind resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives. PiBind resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.
• Specificity: Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds.
  
  PiBind(TM) resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives. PiBind(TM) resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.

Applications/Dilutions

• Application Notes: The resin will bind ATP, making it unavailable for any ATPase enzyme.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

PiBind resin has an extremely high affinity for inorganic phosphate, and is used to eliminate contaminating Pi from buffer solutions to reduce background signals in phosphatase or ATPase assays. PiBind resin may also be used to remove Pi from cell and tissue extracts and is a rapid alternative to desalting or dialysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for PiBind Resin (501-0015)(10)

Publications using 501-0015

• Jung YS, Jun S, Kim MJ et al. TMEM9 promotes intestinal tumorigenesis through vacuolar-ATPase-activated Wnt/b-catenin signalling. Nat. Cell Biol. Dec 1 2018 [PMID: 30374053] (In vitro, Human)
• Andres MT, Acosta-Zaldivar M, Fierro JF. Antifungal mechanism of action of lactoferrin: Identification of H+-ATPase (P3A-type) as a new apoptotic-cell membrane receptor. Antimicrob Agents Chemother. 2016 Jun 20 [PMID: 27139463]
• Contreras CJ, Segvich DM, Mahalingan K et al. Incorporation of phosphate into glycogen by glycogen synthase. Arch Biochem Biophys. 2016 May 1 [PMID: 27036853]
• Park CS, Valomon A, Welzl H3. Integrative Transcriptome Profiling of Cognitive Aging and Its Preservation through Ser/Thr Protein Phosphatase Regulation. PLoS One. 2015 Jun 23 [PMID: 26102285]
• Andres MT, Fierro JF. Antimicrobial Mechanism of Action of Transferrins: Selective Inhibition of H+-ATPase. Antimicrob Agents Chemother. 2010 [PMID: 20625147]
• Graff J, Koshibu K, Jouvenceau A et al. Protein phosphatase 1-dependent transcriptional programs for long-term memory and plasticity. Learn Mem 2010 [PMID: 20592054]
• Wang Y, Liang D, Wang S et al. Role of the G-protein and tyrosine kinase-Rho/ROK pathways in 15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in hypoxic rats. J Biochem 2010 [PMID: 20139061]
• Koshibu K, Graff J, Beullens M et al. Protein Phosphatase 1 Regulates the Histone Code for Long-Term Memory. J Neurosci 2009 [PMID: 19828821]
• Mei J, Wood C, L'abbe MR, Gilani GS, Cooke GM, Curran IH, Xiao CW. Consumption of soy protein isolate modulates the phosphorylation status of hepatic ATPase/ATP synthase beta protein and increases ATPase activity in rats. J. Nutr. 137: 2029-2035. 2007 [PMID: 17709438]
• Racay P, Gregory P, Schwaller B. Parvalbumin deficiency in fast-twitch muscles leads to increased 'slow-twitch type' mitochondria, but does not affect the expression of fiber specific proteins. 2006 Jan. [PMID: 16367751]

Review for PiBind Resin (501-0015) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Hilary Frase ELISA Other 06/08/2012

Summary

• Application: ELISA
• Sample Tested: purified compound
• Species: Other
• Lot: 2818
• Comments: I used this product to remove residual phosphate from ATP and GTP stocks. After 2 treatments of the stock, I was able to remove most of the phosphate, allowing me to use it with the Pi ColorLock Gold reagent. My problem with this product is that it appears to bind the nucleotide as well as the Pi and thus I lost as much as 80% of my original nucleotide. I wanted to use the nucleotides at higher concentrations in the assay, but was unable to due to this limitation.

Blocking

• Blocking Details: n/a

Primary Anitbody

• Dilution Ratio: n/a

Details

• Detection Notes: Pi ColorLock Gold
• ELISA Plate Details: n/a
• Wash Description: n/a

Comments

• Comments: I used this product to remove residual phosphate from ATP and GTP stocks. After 2 treatments of the stock, I was able to remove most of the phosphate, allowing me to use it with the Pi ColorLock Gold reagent. My problem with this product is that it appears to bind the nucleotide as well as the Pi and thus I lost as much as 80% of my original nucleotide. I wanted to use the nucleotides at higher concentrations in the assay, but was unable to due to this limitation.

FAQs for PiBind Resin (501-0015). (Showing 1 - 1 of 1 FAQs).

1. How do I determine how much PiBind resin to use?
   • Without adding the ATP, carry out the assay as described in the protocol.You should then be able to quantify the amount of phosphate contamination. Once you have done this, you can calculate the amount of PiBind that would be necessary to purify the sample.