Recombinant E. coli phrB His Protein

SDS-Page: Recombinant E. coli phrB Protein [NBP2-22657]

• Reactivity: Ec
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant E. coli phrB His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-472 of E.coli phrB
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTTHLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM SPRQAELINA QLNGLQIALA EKGIPLLFRE VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: phr
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 56.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 50% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant E. coli phrB His Protein

• deoxyribodipyrimidine photolyase, FAD-binding
• phr deoxyribodipyrimidine photolyase, FAD-binding

Background

Deoxyribodipyrimidine photolyase, also known as phrB, is involved in repair of UV radiation-induced DNA damage. This protein catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Recombinant E.coli phrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for phrB Recombinant Protein (NBP2-22657)(2)

Publications using NBP2-22657

• Jin SG, Johnson J, Pfeifer GP Circle Damage Sequencing for Whole-Genome Analysis of DNA Damage Methods in molecular biology (Clifton, N.J.) 2023-05-16 [PMID: 37191802]
• Jin S, Pettinga D, Johnson J, Pfeifer G Mapping of DNA damage genome-wide at nucleotide resolution by circle damage sequencing (CD-seq) bioRxiv 2020-06-30

Bioinformatics

• Gene Symbol: phr