Phosphoserine Antibody [HRP]

Images

 
Western Blot: Phosphoserine Antibody [HRP] [NB100-1981] - Phosphoserine Antibody [NB100-1981] - Western blot analysis of human MMRU melanoma cells probed with Phosphoserine polyclonal antibody. Top (from the left): MMRU ...read more

Product Details

Summary
Product Discontinued
View other related Phosphoserine Primary Antibodies

Order Details


    • Catalog Number
      NB100-1981
    • Availability
      Product Discontinued

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Phosphoserine Antibody [HRP] Summary

Immunogen
Phosphoserine conjugated to KLH, and phosvitin mixture
Specificity
Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Knockdown Validated
Readout System
Publications
Read Publication using
NB100-1981 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
HRP stabilizing buffe rcontains PBS, 0.1% BSA, 50% Glycerol
Preservative
0.01% Proclin
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Phosphoserine Antibody [HRP]

  • Phosphoserine; Phosphothreonine

Background

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phosphoserine Antibody (NB100-1981)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Phosphoserine Products

Array NB100-1981

Research Areas for Phosphoserine Antibody (NB100-1981)

Find related products by research area.

Blogs on Phosphoserine.

Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.

Phosphotyrosine: A Global Player in Human Health and Disease
Protein tyrosine phosphorylation is a fundamental mechanism for controlling many aspects of cellular processes, as well as aspects of human health and diseases. Compared with phosphoserine and phosphothreonine, phosphotyrosine signaling is more tightl...  Read full blog post.

New Techniques Using Phosphoserine Antibodies
Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin...  Read full blog post.

Phosphotyrosine: Affecting Locally Acting Globally
Over the past two decades, it has become clear that tyrosine phosphorylation plays a pivotal role in a variety of important signaling pathways in multicellular organisms. In the typical vertebrate cell, phosphotyrosine represents only a tiny fraction ...  Read full blog post.

Phosphoserine: A Basic Cellular Signal with Wide Ranging Effects
Phosphoserine is an ester of serine and phosphoric acid which results from posttranslational modifications. Phosphorylation is a key post-translational modification necessary for normal cellular signaling. Phosphorylated proteins mediate cell division...  Read full blog post.

Phosphotyrosine is Critical Signal Transduction and Regulation
Phosphotyrosine is the phosphorylated version of the amino acid tyrosine, which results from the activation of intracellular protein kinases (e.g. via growth factors) during normal growth and development, well as in transformation and oncogenesis. Pho...  Read full blog post.

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