Phospholipid Assay Kit (Colorimetric/Fluorometric)

Phospholipid Assay Kit (Fluorometric) [KA1635] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

• Reactivity: Hu, Mu, Rt, Po, Ye
• Applications: Func, NULL
• Suitable Sample Type: Plasma (no EDTA), Serum
• Standard Curve Range: Colorimetric Assay: 3 to 200 uM, Fluorimetric Assay: 0.6 to 20 uM
• Sensitivity: OD, FL: 3, 0.6 uM

Phospholipid Assay Kit (Colorimetric/Fluorometric) Summary

• Description: Quality control test: Standard Curve Phospholipid Assay Kit is a quantitative colorimetric/fluorimetric phospholipid determination.
• Specificity: This kit can be used with both colorimetric and fluorometric detection systems.
• Standard Curve Range: Colorimetric Assay: 3 to 200 uM, Fluorimetric Assay: 0.6 to 20 uM
• Sensitivity: OD, FL: 3, 0.6 uM
• Assay Type: Colorimetric/Fluorometric
• Sample Volume: 20 uL
• Kit Type: Assay Kit (Colorimetric/Fluorometric)

Applications/Dilutions

• Dilutions:
  • Functional
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570 nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Phospholipid Kit (KA1635)(10)

Publications using KA1635

• Amira M Effect of Omega-3 or Omega-6 Dietary Supplementation on Testicular Steroidogenesis, Adipokine Network, Cytokines, and Oxidative Stress in Adult Male Rats. Oxid Med Cell Longev. 2021-06-28 [PMID: 34257810]
• Meng-Hsin W, Ai-Ci C, Ling-Hsien T et al. Role of lysine residue of islet amyloid polypeptide in fibril formation, membrane binding, and inhibitor binding. Biochimie. 2020-08-26 [PMID: 32860895]
• Sobhan A, Bahram F, Sally M et al. Changes in lipid biology during ovarian development in farmed beluga sturgeon, Huso huso L. Am J Physiol Regul Integr Comp Physiol. 2020-08-05 [PMID: 32755464]
• Chen Y, Hu K, Huang B et al. Inhibiting Human Calcitonin Fibril Formation with Its Most Relevant Aggregation-Resistant Analog. J Phys Chem B. 2019-11-22 [PMID: 31692350]
• Alfawaz H, Bhat RS, Al-Mutairi M et al. Comparative study on the independent and combined effects of omega-3 and vitamin B12 on phospholipids and phospholipase A2 as phospholipid hydrolyzing enzymes in PPA-treated rats as a model for autistic traits. Lipids Health Dis 2018-08-31 [PMID: 30170600]
• Storti F, Raphael G, Griesser V et al. Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells. Exp Eye Res 2017-09-21 [PMID: 28943268]
• Teo WS, Ling H, Yu AQ, Chang MW. Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid short- and branched-chain alkyl esters biodiesel. Biotechnol Biofuels. 2015-11-04 [PMID: 26543501]
• Getty CM, Dilger RN. Moderate Perinatal Choline Deficiency Elicits Altered Physiology and Metabolomic Profiles in the Piglet. PLoS One 2015-01-01 [PMID: 26196148]
• Feng X, Lian J, Zhao H. Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production. Metab Eng. 2014-10-28 [PMID: 25466225]
• Rossger K, Charpin-El-Hamri G, Fussenegger M. A closed-loop synthetic gene circuit for the treatment of diet-induced obesity in mice. Nat Commun. 2013-01-01 [PMID: 24281397]