Phospho-Tyrosine Antibody

Product Discontinued

Phospho-Tyrosine Antibody Summary

• Immunogen: Affinity purified native tyrosine phosphorylated proteins
• Specificity: Recognizes human phosphotyrosine. Specific to phosphotyrosine containing proteins. This antibody does not recognize non-phosphorylated tyrosine. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:10000
  • Immunoprecipitation 2 - 5 ug
  • Western Blot 1:1000 - 1:2000
• Control:

  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Phospho-Tyrosine Antibody

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Phospho-Tyrosine Antibody (NB110-15088) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/08/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell
• Species: Human

FAQs for Phospho-Tyrosine Antibody (NB110-15088). (Showing 1 - 1 of 1 FAQ).

1. Does NB110-15088 cross react with non phosphorylated proteins ?
   • NB110-15088 is specific to and recognizes only the phosphorylated tyrosine protein. It will not bind to non-phosphorylated protein targets.