Phospho-Tyrosine Antibody (PY20) [HRP]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, ICC
Clone
PY20
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Phospho-Tyrosine Antibody (PY20) [HRP] Summary

Immunogen
Phosphotyrosine was used as the immunogen for the Phosphotyrosine PY20 antibody.
Localization
Depends upon the location of phosphorylated target
Specificity
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:1000-1:4000
  • Immunocytochemistry
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Stock solution in 50% Glycerol/50% PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A or G purified

Alternate Names for Phospho-Tyrosine Antibody (PY20) [HRP]

  • 2 amino 3(4 hydroxyphenyl) propanoic acid
  • 4 hydroxyphenylalanine
  • PhosphoTyrosine
  • pTyrosine
  • Tyrosine

Background

Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.1-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Phospho-Tyrosine Products

Phospho-Tyrosine NBP1-26558

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