Phospho-Tyrosine Antibody (PY20) [HRP]


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Product Details

Reactivity AllSpecies Glossary
Applications WB, ELISA, ICC
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Phospho-Tyrosine Antibody (PY20) [HRP] Summary

Phosphorylated tyrosine and phenylphosphate
Phosphorylated tyrosine and phenylphosphate
IgG2b Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Stock solution in 50% Glycerol/50% PBS (pH 7.4)
No Preservative
Protein A or G purified


  • Western Blot 2 ug/ml
  • ELISA 1:1000-1:4000
  • Immunocytochemistry
Readout System

Reactivity Notes

This antibody detects a chemical and is not species specific.

Alternate Names for Phospho-Tyrosine Antibody (PY20) [HRP]

  • 2 amino 3(4 hydroxyphenyl) propanoic acid
  • 4 hydroxyphenylalanine
  • phosphotyrosine
  • pTyrosine
  • Tyrosine


Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.1-3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Additional Phospho-Tyrosine Antibody (PY20) [HRP] Products

Phospho-Tyrosine NBP1-26558

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