Phospho-Tyrosine Antibody (G104)

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Western Blot: Phospho-Tyrosine Antibody (G104) [NBP1-47562] - analysis of Human A431 cell lysates showing detection of Phosphotyrosine protein using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104. Load: 15 ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
G104
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Phospho-Tyrosine Antibody (G104) Summary

Immunogen
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
Specificity
Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of Phosphotyrosine Antibody was sufficient for detection of phosphorylated tyrosine residues in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary Antibody.
Publications
Read Publications using NBP1-47562.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Phospho-Tyrosine Antibody (G104)

  • 2 amino 3(4 hydroxyphenyl) propanoic acid
  • 4 hydroxyphenylalanine
  • PhosphoTyrosine
  • pTyrosine
  • Tyrosine

Background

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phospho-Tyrosine Antibody (NBP1-47562)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Phospho-Tyrosine Antibody (NBP1-47562) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-47562AF405
Alexa Fluor 488 Labeled NBP1-47562AF488
Alexa Fluor 647 Labeled NBP1-47562AF647
Alexa Fluor 700 Labeled NBP1-47562AF700
DyLight 350 Labeled NBP1-47562UV
DyLight 405 Labeled NBP1-47562V
DyLight 488 Labeled NBP1-47562G
DyLight 680 Labeled NBP1-47562FR
DyLight 755 Labeled NBP1-47562IR

Additional Phospho-Tyrosine Products

Phospho-Tyrosine NBP1-47562

Research Areas for Phospho-Tyrosine Antibody (NBP1-47562)

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